pBW101ParsR-gfp
(Plasmid
#78636)
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PurposepSB3K3 carrying J101-rbs32-arsR-t-ParsR-30gfp-t
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Depositing Lab
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Depositing OrganizationUniversity of Edinburgh
Located in United Kingdom of Great Britain and Northern Ireland -
Citations
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 78636 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
| DNA | 78636-D50 | 50 μg of DNA in Tris buffer | $495 | ||
| DNA | 78636-D100 | 100 μg of DNA in Tris buffer | $585 | ||
Backbone
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Vector backbonepSB3K3
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Backbone manufacturerBioBrick vector, IGEM registry
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Vector typeSynthetic Biology
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameJ101-rbs32-arsR-t-ParsR-30gfp-t
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SpeciesSynthetic
- Promoter see insert
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer pBRforEco
- 3′ sequencing primer RNAde-BB-2R
- (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
DNA (Catalog # 78636-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 78636-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pBW101ParsR-gfp was a gift from Baojun Wang (Addgene plasmid # 78636 ; http://n2t.net/addgene:78636 ; RRID:Addgene_78636) -
For your References section:
Engineering modular and tunable genetic amplifiers for scaling transcriptional signals in cascaded gene networks. Wang B, Barahona M, Buck M. Nucleic Acids Res. 2014 Aug;42(14):9484-92. doi: 10.1093/nar/gku593. Epub 2014 Jul 16. 10.1093/nar/gku593 PubMed 25030903
Map uploaded by the depositor.
