|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||40782||Standard format: Plasmid sent in bacteria as agar stab||1||$85|
This material is available to academics and nonprofits only.
Vector backbonep15a, CamR, phagemid
Vector typeBacterial Expression, Synthetic Biology ; phagemid
Growth in Bacteria
Bacterial Resistance(s)Chloramphenicol, 25 μg/mL
Growth instructionsThis plasmid will turn the strain carrying it a deep purple (more purple the older the colonies are).
Copy numberHigh Copy
Insert Size (bp)7345
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
For detailed composition information, look up the backbone (BBa_J72114) and insert (BBa_J72090) on the Registry of Standard Biological Parts (partsregistry.org).
Please note that Addgene's sequencing results indicate the VioD gene is absent from this construct.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:BBa_J72214-BBa_J72090 was a gift from Christopher Anderson (Addgene plasmid # 40782 ; http://n2t.net/addgene:40782 ; RRID:Addgene_40782)
For your References section:Scalable plasmid transfer using engineered P1-based phagemids. Kittleson JT, DeLoache W, Cheng HY, Anderson JC. ACS Synth Biol. 2012 Dec 21;1(12):583-9. doi: 10.1021/sb300054p. Epub 2012 Aug 30. 10.1021/sb300054p PubMed 23656280