pCEPO2Ya5rescue
(Plasmid #78656)

• Purpose: episomal vector expressing L1 ORF2p to generate tagged Alu inserts suitable for rescue of inserts
• Depositing Lab: Astrid Roy-Engel
• Publication: Ade et al Methods Mol Biol. 2016;1400:183-201. doi: 10.1007/978-1-4939-3372-3_13.

Backbone

• Vector backbone: pCEP4.1
• Backbone manufacturer: Thermo Scientific
• Backbone size w/o insert (bp): 9065
• Total vector size (bp): 16348
• Vector type: Mammalian Expression ; Episomal
• Selectable markers: Neomycin (select with G418), Hygromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert 1

• Gene/Insert name: Ya5rescue cassette
• Alt name: Alurescue
• Species: H. sapiens (human)
• Insert Size (bp): 2504

Gene/Insert 2

• Gene/Insert name: CMV-ORF2p-pA
• Alt name: L1 ORF2opt
• Species: H. sapiens (human)
• Insert Size (bp): 4737
• Mutation: L1 ORF2 codons have been "humanized"
• Tag / Fusion Protein:
  • myc-his (C terminal on insert)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Only when the Alu retrotransposes will the cells become neomycin resistant