pDONR221-OSF-TRIM5-21R(1-300)
(Plasmid #79029)

• Purpose: Gateway entry vector for cloning and propagation in DH5alpha
• Depositing Lab: Wesley Sundquist
• Publication: Li et al Elife. 2016 Jun 2;5. pii: e16269. doi: 10.7554/eLife.16269.

Backbone

• Vector backbone: pDONR221
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 4762
• Total vector size (bp): 3623
• Modifications to backbone: The ccdB cassette in the backbone was replaced with the insert
• Vector type: Insect Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: TRIM5-21R (1-300)
• Alt name: TRIM5-21R (deltaSPRY)(1-300)
• Species: Synthetic
• Insert Size (bp): 1050
• Promoter: T7
• Tag / Fusion Protein:
  • OneSTrEP-FLAG (N terminal on insert)

Cloning Information

• Cloning method: Gateway Cloning
• 5′ sequencing primer: GTTTTCCCAGTCACGAC
• 3′ sequencing primer: CAGGAAACAGCTATGAC

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry