pBABE-3xFLAG-NLS-TPP1-OB
(Plasmid #79060)

• Purpose: Retroviral expression of 3xFLAG tagged, nuclear localized wild-type TPP1-OB folding domain
• Depositing Lab: Steven Artandi
• Publication: Zhong et al Cell. 2012 Aug 3;150(3):481-94. doi: 10.1016/j.cell.2012.07.012.

Backbone

• Vector backbone: pBABE-3xFLAG-NLS
• Backbone manufacturer: Franklin Zhong
• Vector type: Mammalian Expression, Retroviral
• Selectable markers: Puromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: TPP1
• Alt name: wild-type TPP1-OB folding domain
• Species: H. sapiens (human)
• Insert Size (bp): 908
• Mutation: Wild-type TPP-OB folding domain
• Entrez Gene: TPP1 (a.k.a. CLN2, GIG1, LPIC, SCAR7, TPP-1)
• Promoter: CMV
• Tags / Fusion Proteins:
  • 3xFLAG (N terminal on backbone)
  • NLS (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: AscI (not destroyed)
• 3′ cloning site: PacI (not destroyed)
• 5′ sequencing primer: T7
• 3′ sequencing primer: BGH reverse

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

TPP1-OB insert was cloned using unique restriction sites, AscI and PacI into a custom-made pBABE-3xFLAG-NLS vector. Epitope tag was part of the vector backbone. See attached files for the complete sequence from the initiating ATG to the stop codon of the 3xFLAG-NLS-TPP1-OB ORF