pGRG36-Kn-PA1-GFP
(Plasmid #79088)

• Purpose: pGRG36-Kn-PA1-GFP is a Kanamycine resistance plasmid used to integrate a GFP expression cassette into the chromosome of E.coli, described in Yang et al. 2016 (Pending publication Pubmed ID)
• Depositing Lab: Frank Rosenzweig
• Publication: Yang et al Appl Environ Microbiol. 2020 Apr 1;86(8). pii: AEM.00051-20. doi: 10.1128/AEM.00051-20. Print 2020 Apr 1.

Backbone

• Vector backbone: pGRG36
• Backbone manufacturer: McKenzie, G. J., Bmc Microbiology, 2006
• Backbone size w/o insert (bp): 12549
• Total vector size (bp): 14877
• Modifications to backbone: Amp marker disrupted, replaced with a Kan marker, PA1 promoter and GFP expression cassette inserted.
• Vector type: Bacterial Expression ; Bacterial chromosome integration and expression of GFP

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 30°C
• Growth Strain(s): DH5alpha
• Growth instructions: maintained at 30C, but cue at 42. See publication for details.
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: GFP
• Species: E.coli
• Insert Size (bp): 717
• Promoter: PA1

Cloning Information

• Cloning method: Gibson Cloning
• 5′ sequencing primer: gagagattaaagaggagaaaggcgaaATGAGTAAAGGAGAAGAACTTT
• 3′ sequencing primer: gcggatccttatggagttggttaCTATTTGTATAGTTCATCCATG

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry