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(Plasmid #79088)


Item Catalog # Description Quantity Price (USD)
Plasmid 79088 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.


  • Vector backbone
  • Backbone manufacturer
    McKenzie, G. J., Bmc Microbiology, 2006
  • Backbone size w/o insert (bp) 12549
  • Total vector size (bp) 14877
  • Modifications to backbone
    Amp marker disrupted, replaced with a Kan marker, PA1 promoter and GFP expression cassette inserted.
  • Vector type
    Bacterial Expression ; Bacterial chromosome integration and expression of GFP

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
  • Growth Strain(s)
  • Growth instructions
    maintained at 30C, but cue at 42. See publication for details.
  • Copy number
    Low Copy


  • Gene/Insert name
  • Species
  • Insert Size (bp)
  • Promoter PA1

Cloning Information

  • Cloning method Gibson Cloning
  • 5′ sequencing primer gagagattaaagaggagaaaggcgaaATGAGTAAAGGAGAAGAACTTT
  • 3′ sequencing primer gcggatccttatggagttggttaCTATTTGTATAGTTCATCCATG
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGRG36-Kn-PA1-GFP was a gift from Frank Rosenzweig (Addgene plasmid # 79088 ; ; RRID:Addgene_79088)
  • For your References section:

    Fitness and Productivity Increase with Ecotypic Diversity among Escherichia coli Strains That Coevolved in a Simple, Constant Environment. Yang DD, Alexander A, Kinnersley M, Cook E, Caudy A, Rosebrock A, Rosenzweig F. Appl Environ Microbiol. 2020 Apr 1;86(8). pii: AEM.00051-20. doi: 10.1128/AEM.00051-20. Print 2020 Apr 1. 10.1128/AEM.00051-20 PubMed 32060029