pC1GFP-Kir7.1
(Plasmid #79118)

• Purpose: Express N-terminal fused Kir7.1 protein in mammalian cells
• Depositing Lab: Bikash Pattnaik
• Publication: Pattnaik et al PLoS One. 2013 Aug 19;8(8):e71744. doi: 10.1371/journal.pone.0071744. eCollection 2013.

Backbone

• Vector backbone: EGFP-C1
• Backbone manufacturer: Clonetech
• Backbone size w/o insert (bp): 4731
• Total vector size (bp): 5787
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Inwardly rectifying potassium channel KCNJ13
• Alt name: Kir7.1
• Species: H. sapiens (human)
• Insert Size (bp): 1080
• Entrez Gene: KCNJ13 (a.k.a. KIR1.4, KIR7.1, LCA16, SVD)
• Promoter: CMV
• Tag / Fusion Protein:
  • GFP (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoR1 (unknown if destroyed)
• 3′ cloning site: BamH1 (unknown if destroyed)
• 5′ sequencing primer: GCT TCG AAT TCC GAC AGC AGT AAT TG
• 3′ sequencing primer: ATC CGG TGG ATC CTT ATT CTG TCA GT

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Origene

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry