PurposeDirected Evolution of Protein-Protein Interactions
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||79207||Standard format: Plasmid sent in bacteria as agar stab||1||$85|
This material is available to academics and nonprofits only.
Backbone manufacturerDavid Liu Lab
- Backbone size w/o insert (bp) 2992
Vector typeBacterial Expression
Growth in Bacteria
Bacterial Resistance(s)Ampicillin, 100 μg/mL
Growth Strain(s)NEB Turbo
Copy numberLow Copy
Gene/Insert namePlacZ-opt (OR1+2+3) luxAB; Ppro1 434cI-SH2ABL1
- Promoter PlacZ-opt (OR1+2+3); Ppro1
- Cloning method Ligation Independent Cloning
- 5′ sequencing primer n/a
- 3′ sequencing primer n/a (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pAB078d9 was a gift from David Liu (Addgene plasmid # 79207 ; http://n2t.net/addgene:79207 ; RRID:Addgene_79207)
For your References section:Continuous evolution of Bacillus thuringiensis toxins overcomes insect resistance. Badran AH, Guzov VM, Huai Q, Kemp MM, Vishwanath P, Kain W, Nance AM, Evdokimov A, Moshiri F, Turner KH, Wang P, Malvar T, Liu DR. Nature. 2016 May 5;533(7601):58-63. doi: 10.1038/nature17938. Epub 2016 Apr 27. 10.1038/nature17938 PubMed 27120167