pAc5.1B-EGFP-DmNot11
(Plasmid #79253)

• Purpose: Expresses EGFP-tagged DmNot11 in S2 cells
• Depositing Lab: Elisa Izaurralde
• Publication: Bawankar et al RNA Biol. 2013 Feb;10(2):228-44. doi: 10.4161/rna.23018. Epub 2013 Jan 9.

Backbone

• Vector backbone: pAc5.1B-EGFP
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 6057
• Total vector size (bp): 6089
• Vector type: Schneider 2 cell Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: DmNot11
• Alt name: C2ORF29
• Species: D. melanogaster (fly)
• Insert Size (bp): 684
• GenBank ID: NM_138030
• Entrez Gene: Not11 (a.k.a. Dmel_CG13567, C2orf29, CG13567, Dmel\CG13567, NOT11)
• Promoter: Ac5 promoter
• Tag / Fusion Protein:
  • EGFP (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: PacI (not destroyed)
• 3′ cloning site: XbaI (not destroyed)
• 5′ sequencing primer: EGFP-forw
• 3′ sequencing primer: Bgh rev primer

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry