PurposeInsulin receptor isoform A with a inter-domain TagBFP tag at extracellular region (between furin-like domain and transmembrane domain). For imaging insulin receptor trafficing.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||79791||Standard format: Plasmid sent in bacteria as agar stab||1||$85 *|
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Vector typeMammalian Expression
Growth in Bacteria
Bacterial Resistance(s)Ampicillin, 100 μg/mL
Copy numberHigh Copy
SpeciesH. sapiens (human)
Entrez GeneINSR (a.k.a. CD220, HHF5)
- Promoter CMV
/ Fusion Protein
- TagBFP extracellular
- Cloning method Restriction Enzyme
- 5′ cloning site unknown (unknown if destroyed)
- 3′ cloning site unknown (unknown if destroyed)
- 5′ sequencing primer CMV-F
- 3′ sequencing primer BGH-rev (Common Sequencing Primers)
Terms and Licenses
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:InsRA-TagBFP was a gift from James Johnson (Addgene plasmid # 79791 ; http://n2t.net/addgene:79791 ; RRID:Addgene_79791)
For your References section:Inter-domain tagging implicates caveolin-1 in insulin receptor trafficking and Erk signaling bias in pancreatic beta-cells. Boothe T, Lim GE, Cen H, Skovso S, Piske M, Li SN, Nabi IR, Gilon P, Johnson JD. Mol Metab. 2016 Feb 10;5(5):366-78. doi: 10.1016/j.molmet.2016.01.009. eCollection 2016 May. S2212-8778(16)00021-1 [pii] PubMed 27110488