GFP-BLM DM
(Plasmid
#80071)
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PurposeExpression of a sumo-mutant form GFP-BLM with K317R and K331R sumo-site mutations
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Depositing Lab
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Depositing OrganizationMemorial Sloan-Kettering Cancer Center
Located in the United States of America -
Citations
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 80071 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
| DNA | 80071-D50 | 50 μg of DNA in Tris buffer | $495 | ||
| DNA | 80071-D100 | 100 μg of DNA in Tris buffer | $585 | ||
Backbone
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Vector backboneEGFP-C1
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Backbone manufacturerCLONTECH
- Backbone size w/o insert (bp) 4731
- Total vector size (bp) 9112
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Modifications to backboneInserted at Sal1 site
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameBLM
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SpeciesH. sapiens (human)
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MutationK317R (AAA>AGA) and K331R (AAA>AGA)
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GenBank IDU39817.1 NP_000048.1
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Entrez GeneBLM (a.k.a. BS, MGRISCE1, RECQ2, RECQL2, RECQL3)
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Tag
/ Fusion Protein
- GFP-BLM (N terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site SalI (not destroyed)
- 3′ cloning site None (unknown if destroyed)
- 5′ sequencing primer not sure
- 3′ sequencing primer not sure
- (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
DNA (Catalog # 80071-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 80071-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
GFP-BLM DM was a gift from Nathan Ellis (Addgene plasmid # 80071 ; http://n2t.net/addgene:80071 ; RRID:Addgene_80071) -
For your References section:
Intra-nuclear trafficking of the BLM helicase to DNA damage-induced foci is regulated by SUMO modification. Eladad S, Ye TZ, Hu P, Leversha M, Beresten S, Matunis MJ, Ellis NA. Hum Mol Genet. 2005 May 15;14(10):1351-65. Epub 2005 Apr 13. 10.1093/hmg/ddi145 PubMed 15829507
