DT40
(Plasmid
#80415)
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PurposeExpresses 40 interrupted CUG repeats in the context of human DMPK genomic segment containing exons 11-15 with repeats inserted at site in exon 15 that contains the repeats
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Depositing Lab
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Depositing OrganizationBaylor College of Medicine
Located in United States of America -
Citations
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 80415 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
| DNA | 80415-D50 | 50 μg of DNA in Tris buffer | $495 | ||
| DNA | 80415-D100 | 100 μg of DNA in Tris buffer | $585 | ||
Backbone
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Vector backbonesp72
- Backbone size w/o insert (bp) 2400
- Total vector size (bp) 5577
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Modifications to backbonethe interrupted repeats contain 20 CTGs with TCGAC interruptions where SalI and XhoI sites were ligated to concatermize the sets of 20 repeats
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namehuman DMPK exons 11-15 with 40 interrupted CTG repeats
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SpeciesH. sapiens (human)
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Insert Size (bp)3253
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Entrez GeneDMPK (a.k.a. DM, DM1, DM1PK, DMK, MDPK, MT-PK)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please note that there are some discrepancies between Addgene's quality control sequences and the depositor's sequence. The depositor noted that these discrepancies do NOT affect plasmid function.
DNA (Catalog # 80415-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 80415-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
DT40 was a gift from Thomas Cooper (Addgene plasmid # 80415 ; http://n2t.net/addgene:80415 ; RRID:Addgene_80415) -
For your References section:
Muscleblind proteins regulate alternative splicing. Ho TH, Charlet-B N, Poulos MG, Singh G, Swanson MS, Cooper TA. EMBO J. 2004 Aug 4;23(15):3103-12. Epub 2004 Jul 15. 10.1038/sj.emboj.7600300 PubMed 15257297
