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Addgene

pOLENTE
(Plasmid #80683)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 80683 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pTNT
  • Backbone manufacturer
    Promega
  • Modifications to backbone
    Gateway reading frame cassette (rfcA) was inserted in multiple cloning site
  • Vector type
    Mammalian Expression, Synthetic Biology ; in vitro transcription and translation in reticulocyte lysate
  • Promoter T7 and SP6

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol and Ampicillin, 25 & 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    ccdB Survival
  • Copy number
    High Copy

Cloning Information

  • Cloning method Gateway Cloning
  • 5′ sequencing primer atttaggtgacactata
  • 3′ sequencing primer GACACGGAAATGTTGAATAC
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pOLENTE was a gift from Nico Dissmeyer (Addgene plasmid # 80683 ; http://n2t.net/addgene:80683 ; RRID:Addgene_80683)