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pE-SUMO CRYBB2
(Plasmid #80749)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 80749 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pE-SUMO
  • Backbone manufacturer
    LifeSensors
  • Backbone size w/o insert (bp) 5761
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    CRYBB2
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    612
  • GenBank ID
    CU013275
  • Entrez Gene
    CRYBB2 (a.k.a. CCA2, CRYB2, CRYB2A, CTRCT3, D22S665)
  • Promoter T7
  • Tags / Fusion Proteins
    • 6xHIS (N terminal on insert)
    • Smt3 SUMO (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BsaI (destroyed during cloning)
  • 3′ cloning site XbaI (destroyed during cloning)
  • 5′ sequencing primer T7
  • 3′ sequencing primer T7 terminator
  • (Common Sequencing Primers)

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    DNASU plasmid HsCD00402598

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Expresses well as a N-terminal SUMO tagged protein with a 6XHis tag on the N-terminus for purification. The SUMO tag can be removed using ulp1 SUMO protease to yield the N-terminal Ala of mature beta B2-cystallin. Both the SUMO tag and ulp1 SUMO protease can then be removed using an NTA resin. ulp1 SUMO protease can be ordered from LifeSensors or expressed using Addgene plasmid 64697.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pE-SUMO CRYBB2 was a gift from Larry David (Addgene plasmid # 80749 ; http://n2t.net/addgene:80749 ; RRID:Addgene_80749)