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pcDNA3-ALK5 K232R
(Plasmid #80878)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 80878 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    ALK5
  • Alt name
    TGFBR1
  • Alt name
    transforming growth factor beta receptor 1
  • Species
    H. sapiens (human)
  • Mutation
    K232R (kinase dead)
  • Entrez Gene
    TGFBR1 (a.k.a. AAT5, ACVRLK4, ALK-5, ALK5, ESS1, LDS1, LDS1A, LDS2A, MSSE, SKR4, TBR-i, TBRI, TGFR-1, tbetaR-I)
  • Promoter CMV
  • Tag / Fusion Protein
    • HA (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer CMV-F
  • 3′ sequencing primer BGH-rev
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA3-ALK5 K232R was a gift from Aristidis Moustakas (Addgene plasmid # 80878 ; http://n2t.net/addgene:80878 ; RRID:Addgene_80878)
  • For your References section:

    Degradation of the tumor suppressor Smad4 by WW and HECT domain ubiquitin ligases. Moren A, Imamura T, Miyazono K, Heldin CH, Moustakas A. J Biol Chem. 2005 Jun 10;280(23):22115-23. Epub 2005 Apr 6. 10.1074/jbc.M414027200 PubMed 15817471