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(Plasmid #81023)


Full plasmid sequence is not available for this item.


Item Catalog # Description Quantity Price (USD)
Plasmid 81023 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.


  • Vector backbone
  • Backbone manufacturer
  • Backbone size w/o insert (bp) 7700
  • Total vector size (bp) 9200
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
  • Growth Temperature
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy


  • Gene/Insert name
  • Alt name
    additional sex combs like 1, transcriptional regulator
  • Species
    H. sapiens (human)
  • Insert Size (bp)
  • Mutation
    Contains ASXL1 amino acids 1-479
  • Entrez Gene
    ASXL1 (a.k.a. BOPS, MDS)
  • Promoter 5’LTR
  • Tag / Fusion Protein
    • 3X FLAG (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoR1 (unknown if destroyed)
  • 3′ cloning site EcoRI (unknown if destroyed)
  • 5′ sequencing primer pLXSN-5 (5'-CCCTTGAACCTCCTCGTTCGACC-3')
  • 3′ sequencing primer IRES-R (5'-GCATTCCTTTGGCGAGAG-3')
  • (Common Sequencing Primers)

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    MSCV-IRES-GFP ASXL1 (1-479) 3x FLAG was a gift from Anjana Rao (Addgene plasmid # 81023 ; ; RRID:Addgene_81023)
  • For your References section:

    Cancer-associated ASXL1 mutations may act as gain-of-function mutations of the ASXL1-BAP1 complex. Balasubramani A, Larjo A, Bassein JA, Chang X, Hastie RB, Togher SM, Lahdesmaki H, Rao A. Nat Commun. 2015 Jun 22;6:7307. doi: 10.1038/ncomms8307. 10.1038/ncomms8307 PubMed 26095772