pCDF-mTET1
(Plasmid #81052)

• Purpose: bacterial expression of murine TET1
• Depositing Lab: Primo Schaer
• Publication: Weber et al Nat Commun. 2016 Mar 2;7:10806. doi: 10.1038/ncomms10806.

Backbone

• Vector backbone: pCDF-Duet-1
• Backbone manufacturer: Novagen
• Backbone size w/o insert (bp): 3780
• Total vector size (bp): 9726
• Modifications to backbone: both MCS were modified to generate NotI and SpeI cloning sites and to remove the N-terminal 6HIS-tag
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Streptomycin, 50 μg/mL
• Growth Temperature: 30°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: TET1
• Species: M. musculus (mouse)
• Insert Size (bp): 6171
• GenBank ID: NM_001253857.1
• Entrez Gene: Tet1 (a.k.a. 2510010B09Rik, Cxxc6, D10Ertd17e, LCX, mKIAA1676)
• Promoter: T7
• Tag / Fusion Protein:
  • 6HIS (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NotI (not destroyed)
• 3′ cloning site: XbaI (destroyed during cloning)
• 5′ sequencing primer: GGATCTCGACGCTCTCCCT
• 3′ sequencing primer: T7_terminal_primer

Resource Information

• A portion of this plasmid was derived from a plasmid made by: cDNA clone was a gift from Prof Radek Skoda, Department of Biomedicine, University of Basel
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry