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pICE-HA-MRE11-H129N
(Plasmid #82034)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 82034 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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DNA 82034-D50 50 μg of DNA in Tris buffer $495
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DNA 82034-D100 100 μg of DNA in Tris buffer $585
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Backbone

  • Vector backbone
    pICE
  • Backbone manufacturer
    Synthetic - Addgene Plasmid #46960
  • Backbone size w/o insert (bp) 5023
  • Total vector size (bp) 7150
  • Modifications to backbone
    HA Tag and cloning sites
  • Vector type
    Mammalian Expression
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    MRE11
  • Alt name
    MRE11A
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    2127
  • Mutation
    Nuclease-dead mutant: H129N MRE11
  • Entrez Gene
    MRE11 (a.k.a. ATLD, HNGS1, MRE11A, MRE11B)
  • Promoter CMV-tet
  • Tag / Fusion Protein
    • HA (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site AgeI (not destroyed)
  • 3′ cloning site KpnI (not destroyed)
  • 5′ sequencing primer CMV-F
  • 3′ sequencing primer BGH-R
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    I.M.A.G.E. clone #5181691

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 50 μg
  • Concentration 1 μg/μL
  • Pricing $495 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 100 μg
  • Concentration 1 μg/μL
  • Pricing $585 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pICE-HA-MRE11-H129N was a gift from Sébastien Britton & Patrick Calsou (Addgene plasmid # 82034 ; http://n2t.net/addgene:82034 ; RRID:Addgene_82034)

  • For your References section:

    Coordinated nuclease activities counteract Ku at single-ended DNA double-strand breaks. Chanut P, Britton S, Coates J, Jackson SP, Calsou P. Nat Commun. 2016 Sep 19;7:12889. doi: 10.1038/ncomms12889. 10.1038/ncomms12889 PubMed 27641979
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