pUC19_BAS1509
(Plasmid #82354)

• Purpose: T7 in vitro transcription template for Bas mRNA
• Depositing Lab: Nils Walter
• Publication: Chatterjee et al Proc Natl Acad Sci U S A. 2021 Apr 20;118(16). pii: 2023426118. doi: 10.1073/pnas.2023426118.

Backbone

• Vector backbone: pUC19
• Backbone size w/o insert (bp): 2700
• Total vector size (bp): 3281
• Vector type: Unspecified

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: BAS1509 hypothetical protein
• Alt name: BAS1509
• Species: Bacillus anthracis str. Sterne
• Insert Size (bp): 625
• GenBank ID: YP_027776.1 NC_005945.1 (1542527..1543012)
• Promoter: T7

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: XbaI (not destroyed)
• 3′ cloning site: HindIII (not destroyed)
• 5′ sequencing primer: tttcccagtcacgacgtt
• 3′ sequencing primer: tgtggaattgtgagcgga

Resource Information

• Supplemental Documents:
  • pUC19_Bas1509 GenBank File

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry