PurposeGateway Donor vector containing ARAF, part of the Target Accelerator Plasmid Collection.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||82900||Standard format: Plasmid sent in bacteria as agar stab||1||$85|
This material is available to academics and nonprofits only.
Vector typeGateway Entry vector
Growth in Bacteria
Bacterial Resistance(s)Spectinomycin, 50 μg/mL
Copy numberHigh Copy
SpeciesH. sapiens (human)
Entrez GeneARAF (a.k.a. A-RAF, ARAF1, PKS2, RAFA1)
- Promoter None
- Cloning method Gateway Cloning
- 5′ sequencing primer M13pUC-fwd
- 3′ sequencing primer M13-Rev (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
CLOSED (stop codon immediately follows insert)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pDONR223_ARAF_WT was a gift from Jesse Boehm & Matthew Meyerson & David Root (Addgene plasmid # 82900 ; http://n2t.net/addgene:82900 ; RRID:Addgene_82900)
For your References section:High-throughput Phenotyping of Lung Cancer Somatic Mutations. Berger AH, Brooks AN, Wu X, Shrestha Y, Chouinard C, Piccioni F, Bagul M, Kamburov A, Imielinski M, Hogstrom L, Zhu C, Yang X, Pantel S, Sakai R, Watson J, Kaplan N, Campbell JD, Singh S, Root DE, Narayan R, Natoli T, Lahr DL, Tirosh I, Tamayo P, Getz G, Wong B, Doench J, Subramanian A, Golub TR, Meyerson M, Boehm JS. Cancer Cell. 2016 Aug 8;30(2):214-28. doi: 10.1016/j.ccell.2016.06.022. Epub 2016 Jul 28. 10.1016/j.ccell.2016.06.022 PubMed 27478040