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(Plasmid #83276)


Item Catalog # Description Quantity Price (USD)
Plasmid 83276 Standard format: Plasmid sent in bacteria as agar stab 1 $75 *

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  • Vector backbone
  • Backbone manufacturer
    OD260 Inc
  • Backbone size w/o insert (bp) 36741
  • Total vector size (bp) 38638
  • Modifications to backbone
    DraIII digested cos from pE3.1 and DraIII digested expression cassette + Kan resistance from pE1.2 were ligated into SfiI digested pAd388
  • Vector type

Growth in Bacteria

  • Bacterial Resistance(s)
  • Growth Temperature
  • Growth Strain(s)
    NEB Stable
  • Copy number


  • Gene/Insert name
    shRNA to BPTF
  • gRNA/shRNA sequence
  • Species
    H. sapiens (human), M. musculus (mouse)
  • Promoter pUC ori

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer ?
  • (Common Sequencing Primers)

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAd388-BPTF-sh28 was a gift from Joe Landry (Addgene plasmid # 83276 ; ; RRID:Addgene_83276)