-
PurposeDonor vector to knocin EGFP into human Brachyury(T) locus
-
Depositing Lab
-
Depositing OrganizationMorgridge Institute for Research
Located in the United States of America -
Citations
-
Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 83344 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
| DNA | 83344-D50 | 50 μg of DNA in Tris buffer | $495 | ||
| DNA | 83344-D100 | 100 μg of DNA in Tris buffer | $585 | ||
Backbone
-
Vector backboneAddgene plasmid: 31938
- Total vector size (bp) 7548
-
Selectable markersPuromycin
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberHigh Copy
Gene/Insert
-
Gene/Insert nameHuman Brachyury knockin homology arms
-
Alt nameT, BRA
-
SpeciesH. sapiens (human)
-
Insert Size (bp)500
-
Entrez GeneTBXT (a.k.a. SAVA, T, TFT)
-
Tag
/ Fusion Protein
- 2A-EGFP
Resource Information
-
A portion of this plasmid was derived from a plasmid made byYes
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
DNA (Catalog # 83344-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 83344-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
T-2A-EGFP-PGK-Puro was a gift from James Thomson (Addgene plasmid # 83344 ; http://n2t.net/addgene:83344 ; RRID:Addgene_83344) -
For your References section:
Single-cell RNA-seq reveals novel regulators of human embryonic stem cell differentiation to definitive endoderm. Chu LF, Leng N, Zhang J, Hou Z, Mamott D, Vereide DT, Choi J, Kendziorski C, Stewart R, Thomson JA. Genome Biol. 2016 Aug 17;17(1):173. doi: 10.1186/s13059-016-1033-x. 10.1186/s13059-016-1033-x [pii] PubMed 27534536
Map uploaded by the depositor.
