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Purposehuman VEGFR2 with V5 and tGFP tag
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Depositing Lab
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Depositing OrganizationUniversity of Iowa
Located in the United States of America -
Citations
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 83436 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
| DNA | 83436-D50 | 50 μg of DNA in Tris buffer | $495 | ||
| DNA | 83436-D100 | 100 μg of DNA in Tris buffer | $585 | ||
Backbone
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Vector backbonepCMV6 Entry
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Backbone manufacturerOrigene
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namehuman VEGFR2
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SpeciesH. sapiens (human)
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Insert Size (bp)4982
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Entrez GeneKDR (a.k.a. CD309, FLK1, VEGFR, VEGFR2)
- Promoter CMV
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Tags
/ Fusion Proteins
- V5 (N terminal on insert)
- turboGFP (C terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site unknown (unknown if destroyed)
- 3′ cloning site unknown (unknown if destroyed)
- 5′ sequencing primer CMV-F
- 3′ sequencing primer hGH-PA-R
- (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Also see Raikwar et al PLoS One. 2014 Nov 11;9(11):e112794. doi: 10.1371/journal.pone.0112794. eCollection 2014.
DNA (Catalog # 83436-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 83436-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
hKDR was a gift from Christie Thomas (Addgene plasmid # 83436 ; http://n2t.net/addgene:83436 ; RRID:Addgene_83436) -
For your References section:
VEGF-A selectively inhibits FLT1 ectodomain shedding independent of receptor activation and receptor endocytosis. Raikwar NS, Shibuya M, Thomas CP. Am J Physiol Cell Physiol. 2018 Aug 1;315(2):C214-C224. doi: 10.1152/ajpcell.00247.2017. Epub 2018 May 2. 10.1152/ajpcell.00247.2017 PubMed 29719170
