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pILGFPE9
(Plasmid #83545)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 83545 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pUC19
  • Vector type
    Yeast Expression
  • Selectable markers
    URA3 ; kluyveromyces lactis in S. cerevisiae

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    alpha-Select
  • Growth instructions
    alpha-Select from BIOLINE (Genotype: F- deoR endA1 recA1 relA1 gyrA96 hsdR17(rk-, mk+) supE44 thi-1 phoA Δ(lacZYA-argF)U169 Φ80lacZΔM15 λ-)
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    TEF1 promoter-yEGFP
  • Species
    S. cerevisiae (budding yeast)
  • Promoter TEF1

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site unknown (unknown if destroyed)
  • 3′ cloning site unknown (unknown if destroyed)
  • 5′ sequencing primer unknown
  • 3′ sequencing primer yGFP-R
  • (Common Sequencing Primers)

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pILGFPE9 was a gift from Claudia Vickers (Addgene plasmid # 83545 ; http://n2t.net/addgene:83545 ; RRID:Addgene_83545)
  • For your References section:

    Dynamic balancing of isoprene carbon sources reflects photosynthetic and photorespiratory responses to temperature stress. Jardine K, Chambers J, Alves EG, Teixeira A, Garcia S, Holm J, Higuchi N, Manzi A, Abrell L, Fuentes JD, Nielsen LK, Torn MS, Vickers CE. Plant Physiol. 2014 Dec;166(4):2051-64. doi: 10.1104/pp.114.247494. Epub 2014 Oct 15. 10.1104/pp.114.247494 PubMed 25318937