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Addgene

recoverin2b
(Plasmid #83815)

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Item Catalog # Description Quantity Price (USD)
Plasmid 83815 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pCRII-TOPO
  • Backbone manufacturer
    invitrogen
  • Backbone size w/o insert (bp) 3973
  • Total vector size (bp) 4549
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    recoverin2b
  • Species
    D. rerio (zebrafish)
  • Insert Size (bp)
    576
  • GenBank ID
    KT325593
  • Entrez Gene
    rcvrn3 (a.k.a. rcv2b, zgc:73075)

Cloning Information

  • Cloning method TOPO Cloning
  • 5′ sequencing primer CACTCAGACAGAAGTCAT
  • 3′ sequencing primer GTAGACCATCATCGCTTG
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    recoverin2b was a gift from Stephan Neuhauss (Addgene plasmid # 83815 ; http://n2t.net/addgene:83815 ; RRID:Addgene_83815)

  • For your References section:

    Recoverin depletion accelerates cone photoresponse recovery. Zang J, Keim J, Kastenhuber E, Gesemann M, Neuhauss SC. Open Biol. 2015 Aug;5(8). pii: 150086. doi: 10.1098/rsob.150086. 10.1098/rsob.150086 PubMed 26246494
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