|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||8387||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 7164
Vector typeMammalian Expression, Cre/Lox ; Beta-gal
Growth in Bacteria
Bacterial Resistance(s)Ampicillin, 100 μg/mL
Copy numberHigh Copy
Gene/Insert namefloxed EGFP
Alt nameloxI EGFP loxII
Insert Size (bp)800
- Cloning method Restriction Enzyme
- 5′ cloning site SmaI (destroyed during cloning)
- 3′ cloning site SalI (not destroyed)
- 5′ sequencing primer na (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
StuI/SalI fragment from pSV-EGFP/beta-gal. Floxed EGFP gene switch cassette driven by CMV.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:p155 pCMV-EGFP/beta-gal was a gift from Jeffrey Green (Addgene plasmid # 8387 ; http://n2t.net/addgene:8387 ; RRID:Addgene_8387)
For your References section:A single vector containing modified cre recombinase and LOX recombination sequences for inducible tissue-specific amplification of gene expression. Kaczmarczyk SJ, Green JE. Nucleic Acids Res 2001 Jun 15;29(12):E56-6. 10.1093/nar/29.12.e56 PubMed 11410679