pEQ1100
(Plasmid #83937)

• Purpose: express eGFP with a C-terminal 6X his tag in eukaryotic cells
• Depositing Lab: Adam Geballe
• Publication: Child et al J Virol. 2006 Oct;80(20):10173-80.

Backbone

• Vector backbone: pcDNA3.1/V5-His TOPO TA
• Modifications to backbone: vector was digested with XhoI and XbaI, and a biotinylation signal was introduced.
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: EGFP
• Promoter: CMV
• Tags / Fusion Proteins:
  • biotinylation signal (C terminal on backbone)
  • V5 (C terminal on backbone)
  • 6xHIS (C terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: HindIII (not destroyed)
• 3′ cloning site: EcoRV (destroyed during cloning)
• 5′ sequencing primer: CMV-F
• 3′ sequencing primer: BGH-rev

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry