SLC35B2-p2A-E2Crimson
(Plasmid
#83941)
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PurposeLentiviral vector expressing SLC35B2-p2A-E2Crimson
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Depositing Lab
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Publication
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 83941 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepLego-IC2
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Vector typeMammalian Expression, Lentiviral
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameSLC35B2
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SpeciesH. sapiens (human)
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Entrez GeneSLC35B2 (a.k.a. HLD26, PAPST1, SLL, UGTrel4)
- Promoter EF1a
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Tag
/ Fusion Protein
- p2A-E2Crimson (C terminal on insert)
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer cgtatataagtgcagtag (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
ORF sequence is codon-optimized
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
SLC35B2-p2A-E2Crimson was a gift from David Sabatini (Addgene plasmid # 83941 ; http://n2t.net/addgene:83941 ; RRID:Addgene_83941) -
For your References section:
A genome-wide CRISPR screen identifies a restricted set of HIV host dependency factors. Park RJ, Wang T, Koundakjian D, Hultquist JF, Lamothe-Molina P, Monel B, Schumann K, Yu H, Krupzcak KM, Garcia-Beltran W, Piechocka-Trocha A, Krogan NJ, Marson A, Sabatini DM, Lander ES, Hacohen N, Walker BD. Nat Genet. 2016 Dec 19. doi: 10.1038/ng.3741. 10.1038/ng.3741 PubMed 27992415
