Purposetarget miRNA159 for destruction in Arabidopsis thaliana
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||84084||Standard format: Plasmid sent in bacteria as agar stab||1||$85|
This material is available to academics and nonprofits only.
Backbone manufacturerGuiliang Tang (Addgene plasmid # 44182)
- Backbone size w/o insert (bp) 8608
Vector typePlant Expression
Growth in Bacteria
Bacterial Resistance(s)Chloramphenicol and Kanamycin, 25 & 50 μg/mL
Copy numberHigh Copy
SpeciesA. thaliana (mustard weed)
Insert Size (bp)2846
- Promoter 2x35S
- Cloning method Restriction Enzyme
- 5′ cloning site PacI (not destroyed)
- 3′ cloning site PacI (not destroyed)
- 5′ sequencing primer CACTATCCTTCGCAAGACCCTTCC
- 3′ sequencing primer CAACACATGAGCGAAACCCTATAA (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
The insert within this plasmid contains a 2x35S promoter, STTM, T35S and CmR. The STTM sequence in the plasmid contains discrepancies compared to the reference sequence. According to the depositing lab, these mutations should not affect plasmid function.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pFGC5941-PacI-ath-STTM159 was a gift from Guiliang Tang (Addgene plasmid # 84084 ; http://n2t.net/addgene:84084 ; RRID:Addgene_84084)
For your References section:Construction of short tandem target mimic (STTM) to block the functions of plant and animal microRNAs. Tang G, Yan J, Gu Y, Qiao M, Fan R, Mao Y, Tang X. Methods. 2012 Oct;58(2):118-25. doi: 10.1016/j.ymeth.2012.10.006. Epub 2012 Oct 23. 10.1016/j.ymeth.2012.10.006 PubMed 23098881