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PurposeSecreted Luciferase reporter for PPARgamma activity
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Depositing Labs
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Depositing OrganizationINSERM U1139, Physiopathologie et pharmacotoxicologie placentaire humaine
Located in France -
Citations
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 84394 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
| DNA | 84394-D50 | 50 μg of DNA in Tris buffer | $495 | ||
| DNA | 84394-D100 | 100 μg of DNA in Tris buffer | $585 | ||
Backbone
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Vector backbonepNL1.3[secNluc]
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Backbone manufacturerPromega
- Backbone size w/o insert (bp) 3200
- Total vector size (bp) 4600
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namePPARG response element
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Alt namePPRE
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Alt nameApoCIII promotor
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SpeciesH. sapiens (human)
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Insert Size (bp)1200
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Entrez GenePPARG (a.k.a. CIMT1, GLM1, NR1C3, PPARG1, PPARG2, PPARG5, PPARgamma)
- Promoter PPRE
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Tag
/ Fusion Protein
- secNluc (C terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site KpnI (not destroyed)
- 3′ cloning site BglII (not destroyed)
- 5′ sequencing primer RVprimer3
- (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
DNA (Catalog # 84394-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 84394-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
PPRE-pNL1.3 was a gift from Severine Degrelle & Thierry Fournier (Addgene plasmid # 84394 ; http://n2t.net/addgene:84394 ; RRID:Addgene_84394) -
For your References section:
New Transcriptional Reporters to Quantify and Monitor PPARgamma Activity. Degrelle SA, Shoaito H, Fournier T. PPAR Res. 2017;2017:6139107. doi: 10.1155/2017/6139107. Epub 2017 Nov 1. 10.1155/2017/6139107 PubMed 29225614
Map uploaded by the depositor.
