pEF-HA-TSAd-d239-74
(Plasmid
#84560)
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Purposemammalian expression of TSAd-d239-74
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Depositing Lab
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 84560 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepEF HA (pEF BOS derivative)
- Total vector size (bp) 6868
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameTSAd
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Alt nameTSAd-d239-74
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SpeciesH. sapiens (human)
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Insert Size (bp)1212
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Mutationdeletion of aa 239-274
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Entrez GeneSH2D2A (a.k.a. F2771, SCAP, TSAD, VRAP)
- Promoter EF1a
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Tag
/ Fusion Protein
- HA (N terminal on backbone)
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer EF1a-F (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pEF-HA-TSAd-d239-74 was a gift from Anne Spurkland (Addgene plasmid # 84560 ; http://n2t.net/addgene:84560 ; RRID:Addgene_84560) -
For your References section:
T cell specific adapter protein (TSAd) interacts with Tec kinase ITK to promote CXCL12 induced migration of human and murine T cells. Berge T, Sundvold-Gjerstad V, Granum S, Andersen TC, Holthe GB, Claesson-Welsh L, Andreotti AH, Inngjerdingen M, Spurkland A. PLoS One. 2010 Mar 18;5(3):e9761. doi: 10.1371/journal.pone.0009761. 10.1371/journal.pone.0009761 PubMed 20305788