pUS212
(Plasmid
#84580)
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Purpose(Empty Backbone) E.coli cloning and expression vector. Has cloning sites downstream of Tet promoter.
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Depositing Lab
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Publication
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 84580 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 * | |
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Backbone
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Vector backbonepUS212
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Chloramphenicol, 25 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameNone
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
A simple tetracycline-inducible expression vector for E.coli, which has restriction sites that are (partially) compatible with iGEM cloning protocols. Derived from iGEM Parts Registry plasmid pSB1C3. Has unique XbaI and EcoRI sites downstream of Tet promoter.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pUS212 was a gift from Nicholas Coleman (Addgene plasmid # 84580 ; http://n2t.net/addgene:84580 ; RRID:Addgene_84580)
