TF101-STTM-miR394
(Plasmid #84813)

• Purpose: Turning off/down miR394 in Soybean
• Depositing Lab: Guiliang Tang
• Publication: Tang et al Methods. 2012 Oct;58(2):118-25. doi: 10.1016/j.ymeth.2012.10.006. Epub 2012 Oct 23.

Backbone

• Vector backbone: TF101
• Backbone manufacturer: Dr. Kang Wang at Iowa State University
• Vector type: Plant Expression
• Selectable markers: Basta

Growth in Bacteria

• Bacterial Resistance(s): Spectinomycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: STTM-miR394
• Species: Synthetic
• Mutation: bp8 is deleted- please see depositor comments below
• Promoter: unknown

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: PacI (unknown if destroyed)
• 3′ cloning site: PacI (unknown if destroyed)
• 5′ sequencing primer: SP6
• 3′ sequencing primer: T7

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please note that bp8 of the provided STTM-miR394 sequence is missing. The depositing lab noted that this missing bp should not affect plasmid function.