PurposeMinimos transposon with Peft-3:GFP:tbb-2 3'UTR and cbr-unc-119 selection. GFP was optimized for C. elegans, contains 2x NLS (SV40/egl-13), and synthetic introns
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||84827||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
Vector typeBacterial Expression, Worm Expression
Growth in Bacteria
Copy numberHigh Copy
Gene/Insert nameC. elegans codon optimized GFP
SpeciesC. elegans (nematode)
- Promoter Peft-3
- Cloning method Gateway Cloning
- 5′ sequencing primer GAACAGCTATGACCATGATTACG
- 3′ sequencing primer M13F (Common Sequencing Primers)
Terms and Licenses
Expression vector with C. elegans codon-optimized GFP under the eft-3 promoter and with the tbb-2 3'UTR.
Cloned into pCFJ150 for compatibility with mosSCI.
Contains cbr-unc-119 rescue fragment.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pCFJ1137 was a gift from Andrew Fire (Addgene plasmid # 84827 ; http://n2t.net/addgene:84827 ; RRID:Addgene_84827)
For your References section:An Abundant Class of Non-coding DNA Can Prevent Stochastic Gene Silencing in the C. elegans Germline. Frokjaer-Jensen C, Jain N, Hansen L, Davis MW, Li Y, Zhao D, Rebora K, Millet JR, Liu X, Kim SK, Dupuy D, Jorgensen EM, Fire AZ. Cell. 2016 Jul 14;166(2):343-57. doi: 10.1016/j.cell.2016.05.072. Epub 2016 Jun 30. 10.1016/j.cell.2016.05.072 PubMed 27374334