pEMdelta
(Plasmid
#8487)
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Depositing Lab
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Publication
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 8487 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepTrc99A
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Backbone manufacturerNEB
- Backbone size w/o insert (bp) 4163
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin and Kanamycin, 100 & 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameminiTN7
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Alt nametnsA
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SpeciesBacteria
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Insert Size (bp)1763
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Entrez GenetnsA (a.k.a. R721_29)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site KpnI? (not destroyed)
- 3′ cloning site BamHI? (not destroyed)
- 5′ sequencing primer na (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pEMdelta was a gift from Nancy Craig (Addgene plasmid # 8487 ; http://n2t.net/addgene:8487 ; RRID:Addgene_8487) -
For your References section:
Tn7 transposition: target DNA recognition is mediated by multiple Tn7-encoded proteins in a purified in vitro system. Bainton RJ, Kubo KM, Feng JN, Craig NL. Cell 1993 Mar 26;72(6):931-43. 10.1016/0092-8674(93)90581-A PubMed 8384534
