Purposeexpression of YFP in mammalian cells
Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||84910||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
Vector backbonepcDNA3.2 DEST
Vector typeMammalian Expression
Growth in Bacteria
- Promoter CMV
- Cloning method Gateway Cloning
- 5′ sequencing primer CMV-F
- 3′ sequencing primer TK-pA-R (Common Sequencing Primers)
Terms and Licenses
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pcDNA3.2 YFP was a gift from Aaron Gitler (Addgene plasmid # 84910 ; http://n2t.net/addgene:84910 ; RRID:Addgene_84910)
For your References section:Ataxin-2 intermediate-length polyglutamine expansions are associated with increased risk for ALS. Elden AC, Kim HJ, Hart MP, Chen-Plotkin AS, Johnson BS, Fang X, Armakola M, Geser F, Greene R, Lu MM, Padmanabhan A, Clay-Falcone D, McCluskey L, Elman L, Juhr D, Gruber PJ, Rub U, Auburger G, Trojanowski JQ, Lee VM, Van Deerlin VM, Bonini NM, Gitler AD. Nature. 2010 Aug 26;466(7310):1069-75. doi: 10.1038/nature09320. 10.1038/nature09320 PubMed 20740007