Purpose(Empty Backbone) expression in Pichia pastoris
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||85077||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size (bp) 3000
Vector typeYeast Expression
- Promoter GAP
Selectable markersNeomycin (select with G418)
Growth in Bacteria
Bacterial Resistance(s)Kanamycin, 50 μg/mL
Copy numberHigh Copy
- Cloning method Restriction Enzyme
- 5′ sequencing primer GAP
- 3′ sequencing primer 3 AOX1 (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pGKB was a gift from Sheng Yang (Addgene plasmid # 85077 ; http://n2t.net/addgene:85077 ; RRID:Addgene_85077)
For your References section:Hygromycin-resistance vectors for gene expression in Pichia pastoris. Yang J, Nie L, Chen B, Liu Y, Kong Y, Wang H, Diao L. Yeast. 2014 Apr;31(4):115-25. PubMed 24822243