Bsrs074-Prpa*
(Plasmid
#85158)
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PurposeInducibile Rpa* Promoter driving sfGFP
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Depositing Lab
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Depositing OrganizationUniversity of California, San Diego (UCSD)
Located in United States of America -
Sequence Information
Full plasmid sequence is not available for this item.
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 85158 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
| DNA | 85158-D50 | 50 μg of DNA in Tris buffer | $495 | ||
| DNA | 85158-D100 | 100 μg of DNA in Tris buffer | $585 | ||
Backbone
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Vector backbonepBjk2992
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DIAL Strain (colE2)
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Copy numberUnknown
Gene/Insert
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Gene/Insert namePrpa*-sfGFP
Resource Information
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please note that this plasmid may require a unique bacterial strain, so make sure to confirm that you can also obtain the appropriate growth strain. Please contact us at [email protected] or contact our distributors if you have any questions.
DNA (Catalog # 85158-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 85158-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
Bsrs074-Prpa* was a gift from Jeff Hasty (Addgene plasmid # 85158 ; http://n2t.net/addgene:85158 ; RRID:Addgene_85158) -
For your References section:
Quorum Sensing Communication Modules for Microbial Consortia. Scott SR, Hasty J. ACS Synth Biol. 2016 Sep 16;5(9):969-77. doi: 10.1021/acssynbio.5b00286. Epub 2016 May 19. 10.1021/acssynbio.5b00286 PubMed 27172092