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pFLAG-CMV2-Hsp27-S15D/S78D
(Plasmid #85185)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 85185 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Hsp27
  • Species
    H. sapiens (human)
  • Mutation
    S15D/S78D, phosphomimicking mutation
  • Entrez Gene
    HSPB1 (a.k.a. CMT2F, HEL-S-102, HMN2B, HS.76067, HSP27, HSP28, Hsp25, SRP27)
  • Promoter CMV
  • Tag / Fusion Protein
    • Flag (N terminal on backbone)

Cloning Information

Resource Information

  • Terms and Licenses
  • Industry Terms
    • Not Available to Industry

Depositor Comments

The pFLAG-CMV2-Hsp27 wild-type plasmid was obtained from Dr. Park and is described by Park KJ, Gaynor RB, Kwak YT. J Biol Chem 2003; 278:35272-35278. PMID:12829720

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pFLAG-CMV2-Hsp27-S15D/S78D was a gift from Ugo Moens (Addgene plasmid # 85185 ; http://n2t.net/addgene:85185 ; RRID:Addgene_85185)