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mCer-TG2-YFP
(Plasmid #85471)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 85471 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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DNA 85471-D50 50 μg of DNA in Tris buffer $495
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DNA 85471-D100 100 μg of DNA in Tris buffer $585
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Backbone

  • Vector backbone
    mCerulean-C1
  • Modifications to backbone
    eYFP insert cloned into BamHI and XbaI sites at the 3' end of the multiple cloning site (MCS).
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    hTG2
  • Alt name
    transglutaminase 2
  • Species
    H. sapiens (human)
  • Entrez Gene
    TGM2 (a.k.a. G(h), TG(C), TGC, hTG2, tTG)
  • Promoter CMV
  • Tags / Fusion Proteins
    • mCerulean (N terminal on backbone)
    • EYFP (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BglII (unknown if destroyed)
  • 3′ cloning site Acc65I (unknown if destroyed)
  • 5′ sequencing primer CMV-F
  • 3′ sequencing primer EGFP-N
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

The hTG2 insert contains V224G and A141T mutations. The depositor states that these mutations do not affect plasmid function.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 50 μg
  • Concentration 1 μg/μL
  • Pricing $495 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 100 μg
  • Concentration 1 μg/μL
  • Pricing $585 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    mCer-TG2-YFP was a gift from Jeffrey Keillor & Ray Truant (Addgene plasmid # 85471 ; http://n2t.net/addgene:85471 ; RRID:Addgene_85471)

  • For your References section:

    Using FLIM-FRET to measure conformational changes of transglutaminase type 2 in live cells. Caron NS, Munsie LN, Keillor JW, Truant R. PLoS One. 2012;7(8):e44159. doi: 10.1371/journal.pone.0044159. Epub 2012 Aug 31. 10.1371/journal.pone.0044159 PubMed 22952912
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