HOXD1 (murine, partial) HIS-tag pET
(Plasmid
#8560)
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Depositing Lab
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Sequence Information
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Please contact us if you would like Addgene to sequence a portion of this plasmid before you request it.
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Full plasmid sequence is not available for this item.
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 8560 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepET28a
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Backbone manufacturerNovagen
- Backbone size w/o insert (bp) 5400
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameHOXD1
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Alt nameHOX4G
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Alt nameHOX4.9
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SpeciesM. musculus (mouse)
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Insert Size (bp)360
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MutationPartial protein containing 10 AA N-terminal to HD and 40 AA C-terminal to HD; makes protein by Western
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GenBank IDX60034
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Entrez GeneHoxd1 (a.k.a. Hox-4.9)
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Tags
/ Fusion Proteins
- T7 (N terminal on backbone)
- His (N terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site BamHI (not destroyed)
- 3′ cloning site XhoI (destroyed during cloning)
- 5′ sequencing primer T7 (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
RT-PCR from murine embryonic tissue
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
HOXD1 (murine, partial) HIS-tag pET was a gift from Corey Largman (Addgene plasmid # 8560 ; http://n2t.net/addgene:8560 ; RRID:Addgene_8560)
Map uploaded by the depositor.
