PurposeSource of Tol2 transposase driven by a mini-CAGS promoter.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||85600||Standard format: Plasmid sent in bacteria as agar stab||1||$85|
This material is available to academics and nonprofits only.
Backbone manufacturerK. Clark
Vector typeMammalian Expression
Growth in Bacteria
Bacterial Resistance(s)Kanamycin, 50 μg/mL
Copy numberHigh Copy
Gene/Insert nameTol2 transposase
- Promoter mini-CAG
- Cloning method Unknown
- 5′ sequencing primer pCAG-F (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pKC-Tol2 was a gift from Karl Clark & Scott Fahrenkrug (Addgene plasmid # 85600 ; http://n2t.net/addgene:85600 ; RRID:Addgene_85600)
For your References section:Enzymatic engineering of the porcine genome with transposons and recombinases. Clark KJ, Carlson DF, Foster LK, Kong BW, Foster DN, Fahrenkrug SC. BMC Biotechnol. 2007 Jul 17;7:42. 10.1186/1472-6750-7-42 PubMed 17640337