pSM1
(Plasmid #85752)

• Purpose: (Empty Backbone) pDONR split marker vector (GenBank accession number: KX904528) for fungal gene deletion. Used with pSM2.
• Depositing Lab: Damien Fleetwood
• Publication: Rahnama et al J Microbiol Methods. 2016 Dec 22. pii: S0167-7012(16)30368-2. doi: 10.1016/j.mimet.2016.12.017.

Backbone

• Vector backbone: pDONR 221
• Backbone manufacturer: Invitrogen
• Backbone size (bp): 4762
• Vector type: Bacterial Expression
• Promoter: N/A

Growth in Bacteria

• Bacterial Resistance(s): Chloramphenicol and Kanamycin, 25 & 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): ccdB Survival
• Copy number: High Copy

Cloning Information

• Cloning method: Gateway Cloning

Resource Information

• Supplemental Documents:
  • pSM1.gb
• A portion of this plasmid was derived from a plasmid made by: Dr Richard Johnson, AgResearch New Zealand

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please note that there are some seqeunce discrepancies between Addgene's quality control sequences and the depositor's genbank file. The depositor noted that these discrepancies do NOT affect plasmid function.