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RanBP2-RB3-4-pET23a
(Plasmid #85769)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 85769 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pET23a
  • Backbone size w/o insert (bp) 3563
  • Total vector size (bp) 5870
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    RanBP2 fragment
  • Alt name
    Nup358
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    2307
  • Mutation
    fragment (amino acids 2304-3062)
  • Entrez Gene
    RANBP2 (a.k.a. ADANE, ANE1, IIAE3, NUP358, TRP1, TRP2)
  • Promoter T7
  • Tag / Fusion Protein
    • His (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NdeI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer pET-RP
  • (Common Sequencing Primers)

Resource Information

  • Terms and Licenses
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    RanBP2-RB3-4-pET23a was a gift from Frauke Melchior (Addgene plasmid # 85769 ; http://n2t.net/addgene:85769 ; RRID:Addgene_85769)
  • For your References section:

    The RanBP2/RanGAP1*SUMO1/Ubc9 complex is a multisubunit SUMO E3 ligase. Werner A, Flotho A, Melchior F. Mol Cell. 2012 May 11;46(3):287-98. doi: 10.1016/j.molcel.2012.02.017. Epub 2012 Mar 29. 10.1016/j.molcel.2012.02.017 PubMed 22464730