RanBP2-RB3-4-pET23a
(Plasmid #85769)

• Purpose: bacterial expression of RanBP2 SUMO E3 ligase fragment (aa 2304-3062) with C-terminal His tag
• Depositing Lab: Frauke Melchior
• Publication: Werner et al Mol Cell. 2012 May 11;46(3):287-98. doi: 10.1016/j.molcel.2012.02.017. Epub 2012 Mar 29.

Backbone

• Vector backbone: pET23a
• Backbone size w/o insert (bp): 3563
• Total vector size (bp): 5870
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: RanBP2 fragment
• Alt name: Nup358
• Species: H. sapiens (human)
• Insert Size (bp): 2307
• Mutation: fragment (amino acids 2304-3062)
• Entrez Gene: RANBP2 (a.k.a. ADANE, ANE1, IIAE3, NUP358, TRP1, TRP2)
• Promoter: T7
• Tag / Fusion Protein:
  • His (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NdeI (not destroyed)
• 3′ cloning site: XhoI (not destroyed)
• 5′ sequencing primer: T7
• 3′ sequencing primer: pET-RP

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry