pH3FS
(Plasmid
#85780)
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PurposeN-ICE plasmid pH3FS
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Depositing Lab
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Publication
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 85780 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepUG6
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Backbone manufacturerEuroscarf
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Vector typeYeast Expression, Cre/Lox
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Selectable markersHygromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameloxP-HphMX-loxP-3FLAG
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SpeciesS. cerevisiae (budding yeast), Synthetic
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Insert Size (bp)1830
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site NotI (not destroyed)
- 3′ cloning site XhoI (not destroyed)
- 5′ sequencing primer T7 promoter
- 3′ sequencing primer CGCGTCAGCGGGTGTTGG (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pH3FS was a gift from Scott Briggs (Addgene plasmid # 85780 ; http://n2t.net/addgene:85780 ; RRID:Addgene_85780) -
For your References section:
N-ICE plasmids for generating N-terminal 3 x FLAG tagged genes that allow Inducible, Constitutive, or Endogenous expression in Saccharomyces cerevisiae. Zhang Y, Serratore ND, Briggs SD. Yeast. 2016 Dec 12. doi: 10.1002/yea.3226. 10.1002/yea.3226 PubMed 27943405
Map uploaded by the depositor.
Map uploaded by the depositor.
