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pAAVS1-Neo-UBC-M2rtTA-H2BmNeon (donorAB-UBC-mNeon)
(Plasmid #85800)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 85800 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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DNA 85800-D50 50 μg of DNA in Tris buffer $495
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DNA 85800-D100 100 μg of DNA in Tris buffer $585
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Backbone

  • Vector backbone
    N/A
  • Backbone size w/o insert (bp) 3961
  • Total vector size (bp) 11319
  • Vector type
    Human Targeting
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    PPP1R12C
  • Alt name
    AAVS1-homol arms T2A-Neo UBC-rtTA-M2 tetO-H2B-mNeon
  • Alt name
    donor AB-UBC-mNeon
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    7358
  • Mutation
    homology arms for knock-in into first intron
  • Entrez Gene
    PPP1R12C (a.k.a. AAVS1, LENG3, MBS85, p84, p85)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site AjuI (not destroyed)
  • 3′ cloning site RruI (not destroyed)
  • 5′ sequencing primer T3
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Plasmid cloned by combining vectors obtained from Hockemeyer and Jaenisch (Addgene #60843) laboratories

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 50 μg
  • Concentration 1 μg/μL
  • Pricing $495 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 100 μg
  • Concentration 1 μg/μL
  • Pricing $585 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAAVS1-Neo-UBC-M2rtTA-H2BmNeon (donorAB-UBC-mNeon) was a gift from Roland Friedel (Addgene plasmid # 85800 ; http://n2t.net/addgene:85800 ; RRID:Addgene_85800)

  • For your References section:

    Gene signatures of quiescent glioblastoma cells reveal mesenchymal shift and interactions with niche microenvironment. Tejero R, Huang Y, Katsyv I, Kluge M, Lin JY, Tome-Garcia J, Daviaud N, Wang Y, Zhang B, Tsankova NM, Friedel CC, Zou H, Friedel RH. EBioMedicine. 2019 Apr 2. pii: S2352-3964(19)30209-9. doi: 10.1016/j.ebiom.2019.03.064. 10.1016/j.ebiom.2019.03.064 PubMed 30952620
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