pCI-neo-Flag-DHHS
(Plasmid
#85813)
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PurposeTo rescue the expression of DHHC5 catalytically inactive mutant in the knockdown cell lines
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 85813 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepCI-neo
- Backbone size w/o insert (bp) 5500
- Total vector size (bp) 7800
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Modifications to backboneinserted 3x Flag tag
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameZDHHC5 C134S mutant
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SpeciesM. musculus (mouse)
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Insert Size (bp)2200
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GenBank IDNM_144887
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Entrez GeneZdhhc5 (a.k.a. 1110032A17Rik, AI451382, Zisp)
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Tag
/ Fusion Protein
- Flag (N terminal on backbone)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCI-neo-Flag-DHHS was a gift from Sandra Hofmann (Addgene plasmid # 85813 ; http://n2t.net/addgene:85813 ; RRID:Addgene_85813) -
For your References section:
Systematic siRNA Screen Unmasks NSCLC Growth Dependence by Palmitoyltransferase DHHC5. Tian H, Lu JY, Shao C, Huffman KE, Carstens RM, Larsen JE, Girard L, Liu H, Rodriguez-Canales J, Frenkel EP, Wistuba II, Minna JD, Hofmann SL. Mol Cancer Res. 2015 Apr;13(4):784-94. doi: 10.1158/1541-7786.MCR-14-0608. Epub 2015 Jan 8. 10.1158/1541-7786.MCR-14-0608 PubMed 25573953
