GST-mDia1(FH1FH2DAD)-His
(Plasmid #85822)

• Purpose: contains the formin homolgy domains: FH2 is required for nucleation, processive tracking of barbed-end of actin filaments ; FH1 permits enhancement of actin polymerization rate.
• Depositing Labs: Antoine Jégou, Guillaume Romet-Lemonne
• Publication: Jegou et al Nat Commun. 2013;4:1883. doi: 10.1038/ncomms2888.

Backbone

• Vector backbone: modified pGEX-6P-1
• Backbone size w/o insert (bp): 5003
• Total vector size (bp): 7067
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Protein diaphanous homolog 1
• Alt name: mDia1 (558-1255) FH1-FH2-DAD
• Species: M. musculus (mouse)
• Insert Size (bp): 2064
• Entrez Gene: Diaph1 (a.k.a. D18Wsu154e, Dia1, Diap1, Drf1, p140mDia)
• Promoter: tac
• Tags / Fusion Proteins:
  • GST (N terminal on backbone)
  • 8*His (C terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (not destroyed)
• 3′ cloning site: XhoI (not destroyed)
• 5′ sequencing primer: CTGGCAAGCCACGTTTGG
• 3′ sequencing primer: GGAGCTGCATGTGTCAGAG

Resource Information

• Supplemental Documents:
  • GST-mDia1 (FH1-FH2-DAD)-His.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry