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pA9HD
(Plasmid #8588)

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Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 8588 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    PET28a+
  • Backbone manufacturer
    Novagen
  • Backbone size w/o insert (bp) 5369
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    HoxA9 190-271with hexapeptide
  • Alt name
    Homeodomain
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    261
  • GenBank ID
    AAB40867
  • Entrez Gene
    HOXA9 (a.k.a. ABD-B, HOX1, HOX1.7, HOX1G)
  • Tag / Fusion Protein
    • His (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NcoI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer T7
  • (Common Sequencing Primers)

Resource Information

Depositor Comments

His tag of vector not expressed: stop codon prior. Best expressed in BL21-CodonPlus(DE3)-RIL cells.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pA9HD was a gift from Cynthia Wolberger (Addgene plasmid # 8588 ; http://n2t.net/addgene:8588 ; RRID:Addgene_8588)
  • For your References section:

    Structure of HoxA9 and Pbx1 bound to DNA: Hox hexapeptide and DNA recognition anterior to posterior. LaRonde-LeBlanc NA, Wolberger C. Genes Dev 2003 Aug 15;17(16):2060-72. 10.1101/gad.1103303 PubMed 12923056