PurposeFLAG-mPar6a K19A - Does not bind to aPKC
Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||86149||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Vector backboneMSCV -PGK-Puro-IRES-GFP
Vector typeMammalian Expression, Retroviral
Growth in Bacteria
Growth Strain(s)NEB Stable
Copy numberHigh Copy
Alt namePar6 alpha
SpeciesM. musculus (mouse)
MutationLysine to Alanine mutation K19A
Entrez GenePard6a (a.k.a. 0710008C04Rik, 2610010A15Rik, PAR-6A, PAR6alpha, Par-6, Par6, Par6c, TAX40, Tip-40)
- Promoter LTR
/ Fusion Protein
- FLAG (N terminal on backbone)
- Cloning method Restriction Enzyme
- 5′ cloning site Hpa1 site (unknown if destroyed)
- 3′ cloning site Hpa1 site (unknown if destroyed)
- 5′ sequencing primer MSCV-F
- 3′ sequencing primer MSCV-R (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:FLAG-mPar6a K19A was a gift from Senthil Muthuswamy (Addgene plasmid # 86149 ; http://n2t.net/addgene:86149 ; RRID:Addgene_86149)
For your References section:The polarity protein Par6 induces cell proliferation and is overexpressed in breast cancer. Nolan ME, Aranda V, Lee S, Lakshmi B, Basu S, Allred DC, Muthuswamy SK. Cancer Res. 2008 Oct 15. 68(20):8201-9. 10.1158/0008-5472.CAN-07-6567 PubMed 18922891
Map uploaded by the depositor.